Nucleotides support growth of selected intestinal bacteria
- Publication Type
- Contribution to conference
- Authors
- Sauer N, Bauer E, Vahjen W, Zentek J and Mosenthin R.
- Year of publication
- 2009
- Published in
- Programme and Books of Abstracts
- Band/Volume
- XI/
- Series/labeling
- International Symposium on Digestive Physiology of Pigs (DPP 2009)
- Page (from - to)
- P. 69 (2.27)
- Conference name
- Digestive Physiology of Pigs
- Conference location
- Montbrió del Camp (Barcelona), Spain
- Conference date
- 20.05.2009
- Keywords
- bacteria, gastrointestinal tract, intestinal bacteria, monogastric animal, Nukleotide
Dietary nucleotides play an important role in the development of the gastrointestinal tract and may promote intestinal bacteria in young monogastrics. Two preliminary in vitro studies have been performed to evaluate the effects of individually added nucleotides (AMP, CMP, GMP, IMP, UMP) in different concentrations on growth of selected bacterial strains, as measured by optical density levels. In the first experiment, a basal medium based on NH4CI was used to test growth of three different Escherichia coli strains (DSM 2840, PS 37, PS 79) using nucleotide concentrations of 0, 0.1, 0.3, 0.8 and 1 mg/ml, respectively. In the second experiment, growth of Lactobacillus reuteri (DSM 20016), L. amylovorus (DSM 20513), Enterococcus faecium (DSM 2146) and E. faecalis (DSM 6134) was assessed using two different media (based on yeast extract or casein) containing graduated levels of the respective nucleotides (0, 0.1, 0.4, and 1 mg/ml). E. coli strains showed diverse responses depending on the type of nucleotide used. In the second experiment, all tested strains were able to utilize the yeast extract medium more efficiently when compared to the casein medium. However, no differences in the efficiency of added single nucleotides at any given concentration could be detected. These preliminary results suggest a potential role of nucleotides in stimulating growth of intestinal bacteria, although this has to be confirmed by expanding the range of bacterial strains to be assessed.