Bioreactor cultivation of Escherichia coli for production of recombinant penicillin G amidase from Alcaligenes faecalis

Publication Type
Journal contribution (peer reviewed)
Authors
Deak, P. M., Lutz-Wahl, S., Bothe, H., Fischer, L.
Year of publication
2003
Published in
Biotechnology Letters
Band/Volume
25/5
DOI
10.1023/A:1022498314354
Page (from - to)
397-400
Abstract

The penicillin G amidase (PGA) from Alcaligenes faecalis, which has interesting properties for use in combinatorial biochemistry, was produced by recombinant expression in Escherichia coli. The corresponding gene was cloned into a multicopy vector under the strict regulatory control of the rhamnose inducible promoter. Cells were grown in a synthetic minimal medium in a bioreactor (5 l working vol.), and production of PGA was induced by repeated addition of the inducer rhamnose, that served also as a carbon source. The fermentation yield was about 4500 units PGA activity per liter of culture medium.

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