Publications: Prof. Dr. rer. nat. Andreas Kuhn

Title	Year	Literature Type	Authors
A natural variant of arylsulfatase from Kluyveromyces lactis shows no formylglycine modification and has no enzyme activity Applied Microbiology and Biotechnology	2018	Journal contribution (peer reviewed)	Stressler, T.; Reichenberger, K.; Glück, C.; Leptihn, S.; Pfannstiel, J.; Swietalski, P.; Kuhn, A., Seitl; I.; Fischer, L.
Each protomer of a dimeric YidC functions as a single membrane insertase Scientific Reports	2018	Journal contribution (peer reviewed)	Spann, D., Pross, E., Y. Chen, R. Dalbey, A. Kuhn
Large conformational changes of a highly dynamic pre-protein binding domain in SecA 03.09.2018 in Communications Biology	2018	Journal contribution (peer reviewed)	Isabel Ernst, Maximilian Haase, Stefan Ernst, Shuguang Yuan, Andreas Kuhn & Sebastian Leptihn
The fascinating biology behind phage display: Filamentous Phage Assembly Mol Microbiol	2018	Journal contribution (peer reviewed)	Loh B., Kuhn A., Leptihn S.
YidC-mediated Membrane Insertion FEMS Microbiology Letters	2018	Journal contribution (peer reviewed)	Kiefer D., Kuhn A.
Assisted and Unassisted Protein Insertion into Liposomes. Biophys Journal	2017	Journal contribution (peer reviewed)	Kuhn A., Haase M., Leptihn S.
Mechanism of the Spontaneous and Directional Membrane Insertion of a 2-Transmembrane Ion Channel ACS Chemical Biology	2017	Journal contribution (peer reviewed)	Steffen Altrichter, Maximilian Haase, Belinda Loh, Andreas Kuhn, Sebastian Leptihn
Membrane protein insertase YidC in bacteria and archaea Molecular Microbiology	2017	Journal contribution (peer reviewed)	Kuhn, Andreas, Kiefer, Dorothee
Targeting and insertion of membrane proteins EcoSal plus 7	2017	Journal contribution (peer reviewed)	Kuhn, A., Koch, H.-G., Dalbey, R. E.
The Transmembrane Morphogenesis Protein gp1 of Filamentous Phages Contains Walker A and Walker B Motifs Essential for Phage Assembly Viruses	2017	Journal contribution (peer reviewed)	Belinda Loh, Maximilian Haase, Lukas Mueller, Andreas Kuhn and Sebastian Leptihn
A conformational crosstalk between SecA and SecY opens the SecYEG channel. Current Biol.	2016	Journal contribution (peer reviewed)	Kuhn, A., Dalbey, R. E.,
A Novel Glutamyl (Aspartyl)-Specific Aminopeptidase A from Lactobacillus delbrueckii with Promising Properties for Application PLOS ONE	2016	Journal contribution (peer reviewed)	Stressler, T.; Ewert, J.; Merz, M.; Funk, J.; Claaßen, W.; Lutz-Wahl, S.; Schmidt, H.; Kuhn, A.; Fischer, L.
Detection, production, and application of microbial arylsulfatases Applied Microbiology and Biotechnology	2016	Journal contribution (peer reviewed)	Stressler, T.; Seitl, I.; Kuhn, A.; Fischer, L.
Homologous expression and biochemical characterization of the arylsulfatase from Kluyveromyces lactis and its relevance in milk processing Applied Microbiology and Biotechnology	2016	Journal contribution (peer reviewed)	Stressler, T.; Leisibach, D.; Lutz-Wahl, S.; Kuhn, A.; Fischer, L.
Membrane targeting and insertion of the C-tail protein SciP. J. Mol. Biol.	2016	Journal contribution (peer reviewed)	Proß, E., Soussoula, L., Seitl, I., Lupo, D., Kuhn, A.
T7 ejectosome assembly: A story unfolds. Bacteriophage	2016	Journal contribution (peer reviewed)	Leptihn S, Gottschalk J, Kuhn A.
Extracellular peptidases from insect- and compost-associated microorganisms: screening and usage for wheat gluten hydrolysis European Food Research and Technology	2015	Journal contribution (peer reviewed)	Stressler, T.; Eisele, T.; Baur, C.; Wangler, J.; Kuhn, A.; Fischer, L.;
Membrane insertases are present in all three domains of life Structure	2015	Journal contribution	Dalbey, R.E.; Kuhn, A.
The T7 ejection nanomachine components gp15-gp16 form a spiral ring complex that binds DNA and a lipid membrane Virology	2015	Journal contribution	Lupo, D.; Leptihn, S.; Nagler, G.; Haase, M.; Molineux, I.; Kuhn, A.
How YidC inserts and folds proteins across a membrane Nature Structural & Molecular Biology	2014	Journal contribution	Dalbey, R.E.; Kuhn, A.
Introduction to a special issue on protein trafficking and secretion Biochimica et Biophysica Acta (BBA)	2014	Other publication	Kuhn, A.
The C-terminal regions of YidC from Rhodopirellula baltica and Oceanicaulis alexandrii bind to ribosomes and partially substitute for SRP receptor function in Escherichia coli Molecular Mircobiology	2014	Journal contribution	Seitl, I.; Wickles, St.; Beckmann, R.; Kuhn, A.; Kiefer, D.
The membrane insertase YidC. Biochimica et Biophysica Acta (BBA)	2014	Other publication	Dalbey, R.E.; Kuhn, A.; Zhu, L.; Kiefer, D.
The polarity and charge of the periplasmic loop determines the YidC and Sec translocase requirement for the M13 procoat protein Journal of Biological Chemistry	2014	Other publication	Soman, R.; Yuan, J.; Kuhn, A.; Dalbey, R.E.
The role of the strictly conserved positively charged residue differs among the Gram-positive, Gram-negative and Chloroplast YidC homologs Journal of Biological Chemistry	2014	Journal contribution	Chen, Y.; Soman, R.; Zhu, L.; Kuhn, A.; Dalbey, R.E.
Production of Polyunsaturated Fatty Acids by Mortierella alpina Using Submerse and Solid State Fermentation Chemie Ingenieur Technik	2013	Journal contribution (peer reviewed)	Stressler, T.; Eisele, T.; Rost, J.; Haunschild, E-M.; Kuhn, A.; Fischer, L.
Real time observation of single membrane protein insertion events by the Escherichia coli insertase YidC PLosOne	2013	Journal contribution (peer reviewed)	Winterfeld S, Ernst S, Börsch M, Gerken U, Kuhn A.
Both YidC and SecYEG are required for translocation of the periplasmic loops 1 and 2 of the multispanning membrane protein TatC. Journal of Molecular Biology	2012	Journal contribution	Zhu, L., Klenner, C., Kuhn, A. and Dalbey R.E.
Dynamic Disulfide Scanning of the Membrane-inserting Pf3 Coat Protein Reveals Multiple YidC Substrate Contacts The Journal of Biological Chemistry	2012	Journal contribution	Klenner, C. and Kuhn, A.
Membrane interaction of the portal protein gp20 of bacteriophage T4 J. Virology	2012	Journal contribution	Quinten, T. and Kuhn, A.
Membrane protein insertion of variant MscLproteins occurs at YidC and SecYEG of E. coli. J. Mol. Biol.	2012	Journal contribution (peer reviewed)	Neugebauer, S. A., Baulig, A., Kuhn, A., Facey, S. J.
Assembly of Bacterial Inner Membrane Proteins Annual Review Biochemistry	2011	Journal contribution	Ross E. Dalbey, Peng Wang, Andreas Kuhn
M13 procoat protein insertion into YidC and SecYEG proteoliposomes and liposomes Journal of Molecular Biology	2011	Journal contribution	Stiegler, N., Dalbey, R. and Kuhn, A.
Membrane insertion and assembly of epitope-tagged gp9 at the tip of the M13 phage BMC Microbiol.	2011	Journal contribution	Ploss, M., and Kuhn, A.
Substrate-dependent conformational dynamics of the Escherichia coli membrane insertase YidC Biochemistry	2011	Journal contribution	Imhof, N., Kuhn, A., Gerken, U.
YidC-driven membrane insertion of single fluorescent Pf3 coat proteins J. Mol. Biol.	2011	Journal contribution	Ernst, S., Schönbauer, AK., Bär, G., Börsch, M. and Kuhn, A.
Biogenesis of bacterial inner-membrane proteins Cellular and Molecular Life Sciences	2010	Journal contribution	Facey, S.J. and Kuhn, A.
Global change of gene expression and cell physiology in YidC-depleted E. coli J. Bacteriology	2010	Journal contribution	Wang, P., Kuhn, A. and Dalbey, R. E.
Kinetics of filamentous phage assembly Physical Biology	2010	Journal contribution	Ploss, M. and Kuhn, A.
Membrane insertion of small proteins Protein Secretion	2010	Book chapter	Kuhn, A., Stiegler, N. and Schubert, A.
Membrane protein insertion in E. coli Protein Secretion	2010	Book chapter	Yuan, R., Dalbey, R. and Kuhn, A.
The photosensorprotein Ppr of Rhodocista centenaria is linked to the chemotaxis signalling pathway BMC Microbiology	2010	Journal contribution	Kreutel, S., Kuhn, A., Kiefer, D.
Alb4 of Arabidopsis promotes assembly and stabilisation of a none chlorophyll-binding photosynthetic complex, the CF1CF0-ATP synthase Molecular Plant	2009	Journal contribution	Benz, M., Bals, T., Yuan, J., Dalbey, R., Kuhn, A., Schünemann, D., Soll, J. and Ankele, E.
From the Sec complex to the membrane insertase YidC Biol. Chem.	2009	Journal contribution	Kuhn, A.
Substrate-induced conformational change of the E. coli membrane insertase YidC Biochemistry	2009	Journal contribution	Winterfeld, S., Imhof, N., Roos, T., Bär, G., Kuhn, A. and Gerken, U.
An amphiphilic region in the cytoplasmic domain of KdpD is recognized by SRP and targeted to the E.coli membrane. Molecular Microbiology	2008	Journal contribution	Maier, K., Hubich, S. , Liebhart, H., Krauss, S., Kuhn, A., Facey, S.
Initial binding process of the membrane insertase YidC with its substrate protein Pf3 coat. Biochemistry	2008	Journal contribution	Gerken, U., Erhardt, D., Bär, G., Ghosh, R., Kuhn, A.
The Pf3 coat protein contacts TM1 and TM3 of YidC during membrane biogenesis FEBS letters	2008	Journal contribution	Klenner, C., Yuan, J., Dalbey, R., Kuhn, A.
Cysteine residues in the transmembrane regions of M13 procoat suggest that oligomeric coat proteins assemble onto phage particles. J. Bacteriol.	2007	Journal contribution	Nagler, C., Nagler, G. and Kuhn, A.
The mechansosensitive channel protein MscL is targeted by SRP to the novel YidC membrane insertion pathway of E. coli. J. Mol. Biol.	2007	Journal contribution	Facey, S., Neugebauer, S., Krauss, S., Kuhn, A.
YidC is an essential and mulitfunctional component in membrane protein assembly. Int. Cytolog. Rev.	2007	Journal contribution	Kiefer, D. and Kuhn, A.
Different regions of the nonconserved large periplasmic domain of Escherichia coli YidC are involved in SecF interaction and in membrane insertase activity. Biochemistry	2006	Journal contribution	Xie, K., Kiefer, D., Nagler, G., Dalbey, R., Kuhn, A.
Membrane biogenesis of subunit II of cytochrome bo oxidase: Contrasting requirements for insertion of N-terminal and C-terminal domains. J. Mol Biol.	2006	Journal contribution	Celebi, N., L. Yi, S. Facey, A. Kuhn, R. Dalbey
Proton-decoupled 15N and 31P solid-state NMR investigations of the Pf3 coat protein in oriented phospholipid bilayers. FEBS J.	2006	Journal contribution	Aisenbrey, C., U. Harzer, G. Bauer-Manz, G. Bär, C. Sizun, A. Kuhn, B. Bechinger
The cytoplasmic C-terminal domain of E.coli KdpD protein functions as a sensor kinase. J. Bacteriol.	2006	Journal contribution	Rothenbücher, M., M. Kossmann, D. Kiefer, S. Facey, A. Kuhn
Identification of the preprotein binding domain of SecA. J. Biol. Chem.	2005	Journal contribution	Papanikou, E., S. Karamanou, C. Baud, M. Frank, G. Sianidis, D. Keramissanou, C. Kalodimos, A. Kuhn, A. Economou
Involvement of SecDF and YidC in the membrane insertion of M13 procoat mutants. Biochemistry	2005	Journal contribution	Chen, M., J. Yuan, L. Yi, K. Xie, S. Facey, N. Pradel, L-F. Wu, A. Kuhn, R.E. Dalbey
Purification of a functional mature region from a SecA-dependent preprotein. Protein Expr. Purif.	2005	Journal contribution	Baud, C., E. Papanikou, S. Karamanou, G. Sianidis, A. Kuhn, A. Economou
Escherichia coli YidC folds Sec-independent proteins into the membrane. Embo J.	2004	Journal contribution	Serek, J., Bauer-Manz, G., Struhalla, G., Vandenberg, L., Kiefer, D., Dalbey, R. and Kuhn, A.
Membrane integration of E.coli model proteins. Biochim.Biophys.Acta	2004	Journal contribution	Facey, S. and Kuhn, A.
YidC family members are involved in the membrane insertion, lateral integration, folding and assembly of membrane proteins. J. Cell Biol.	2004	Journal contribution	Dalbey, R.E. and Kuhn, A.
Defining the regions of the Escherichia coli YidC that contribute to activity. J. Biol. Chem.	2003	Journal contribution	Jiang, F., M. Chen, J.-W. de Gier, A. Kuhn and R. Dalbey
The Alb3/Oxa1/YidC protein family: membrane chaperones facilitating membrane insertion? Trends in Cell Biology	2003	Journal contribution	Kuhn, A., R. Stuart, R. Henry and R. Dalbey
The sensor protein KdpD inserts into the Escherichia coli membrane independent of the Sec translocase and YidC. Eur. J. Biochem.	2003	Journal contribution	Facey, S. and A. Kuhn
Direct interaction of YidC with the Sec-independent Pf3 coat protein during its membrane insertion. J. Biol. Chem.	2002	Journal contribution	Chen, M., J. Samuelson, F. Jiang, M. Müller, A. Kuhn and R. Dalbey
Membrane protein insertion into bacterial membranes and the endoplasmic reticulum. Protein targeting, transport and translocation	2002	Book chapter	Kuhn, A. and Spiess, M
Anionic lipids stimulate Sec-independent insertion of a membrane protein lacking charged amino acid side chains. EMBOreports	2001	Journal contribution	Ridder, A., Kuhn, A., Killian, A., de Kruijff, B.
Fluorescence energy transfer shows a close helix-helix distance in the transmembrane M13 procoat protein. Biochemistry	2001	Journal contribution	Eisenhawer, M., Cattarinussi, S., Kuhn, A., Vogel, H.
Function of YidC for the insertion of M13 procoat protein in E.coli: Translocation of mutants that show differences in their membrane potential dependence and Sec-requirement. J.Biol.Chem.	2001	Journal contribution	Samuelson, J.C., Jiang, F., Yi, L., Chen, M., Kuhn, A., Dalbey, R.E.
Indecisive M13 procoat mutants bind to SecA but do not activate the translocation ATPase. J.Biol.Chem.	2001	Journal contribution	Roos, T., Kiefer, D., Hugenschmidt, S., Economou, A., Kuhn, A.
Evolutionarily related insertion pathways of bacterial, mitochondrial and thylakoidal membrane proteins. Ann.Rev.Cell.Dev.Biology	2000	Journal contribution	Dalbey, R., Kuhn, A.
The topological role of interfacial tryptophans in single-spanning membrane proteins. Biochemistry	2000	Journal contribution	Ridder, A., Morein, S., Stam, J., Kuhn, A., Killian, A., de Kruijff, B.
YidC mediates both Sec-dependent and Sec-independent membrane protein insertion. Nature	2000	Journal contribution	Samuelson, J.C., Chen, M., Jiang, F., Möller, I., Wiedmann, M., Kuhn, A., Phillips, G.J., Dalbey, R.
A molecular switch in SecA protein couples ATP hydrolysis to protein translocation. Mol. Microbiol.	1999	Journal contribution	Karamanou, S., Vrontou, E., Sianidis, G., Baud, C., Roos, T., Kuhn, A., Politou, S., Economou, A.
Hydrophobic forces drive the spontaneous membrane insertion of the bacteriophage Pf3 coat protein without topological control EMBO J.	1999	Journal contribution	Kiefer, D., Kuhn, A.
Differential use of the signal recognition particle translocase pathway for inner membrane protein assembly in Escherichia coli. Proc. Natl. Acad. Sci. U.S.A.	1998	Journal contribution	De Gier, J. W. L., Scotti, P.A., Saaf, A., Valent, Q. A., Kuhn, A., Luirink, J., von Heijne, G.
Insertion of single and multi-spanning proteins into the bacterial cytoplasmic membrane Protein Targeting and Translocation	1998	Book chapter	Kiefer, D., Kuhn, A.
Negatively charged amino acid residues determine the membrane orientation of the Sec independent Pf3 coat protein. EMBO J.	1997	Journal contribution	Kiefer, D.; Hu, X.; Dalbey, R.E. and Kuhn, A.
Thermodynamics of the membrane insertion process of the M13 procoat protein: A bilayer-traversing protein comprising a leader sequence. Biochemistry	1996	Journal contribution	Soekarjo, M.; Kuhn, A. and Vogel, H.
Bacteriophage Pf3 and M13 as model systems for Sec-independent protein transport. FEMS Microbiol. Rev.	1995	Journal contribution	Kuhn, A.
Directionality in protein translocation across membranes Trends Cell Biol.	1995	Journal contribution	Dalbey, R.E., Kuhn, A., von Heijne, G.
Membrane insertion of small proteins. Functional and evolutionary aspects. Protein export and membrane biogenesis	1995	Book chapter	Kiefer, D., Kuhn, A.
The translocation of negatively charged residues across the membrane is driven by the electrochemical potential. Evidence for an electrophoresis-like membrane transfer mechanism. EMBO J.	1995	Journal contribution	Cao, G.; Kuhn, A. and Dalbey, R.E.
A dual role for phosphatidyl glycerol in protein translocation across the Escherichia coli inner membrane. J. Biol. Chem.	1994	Journal contribution	Kusters, R.; Breukink, E.; Gallusser, A.; Kuhn, A. and de Kruijff, B.
Evidence for a loop-like insertion mechanism of pro-OmpA into the inner membrane of Escherichia coli. Eur. J. Biochem.	1994	Journal contribution	Kuhn, A.; Kiefer, D.; Köhne, C.; Zhu, H.-Y., Tschantz, W. and Dalbey, R.E.
Pf3 coat protein forms voltage-dependent ion channels in planar lipid bilayers. Biochemistry	1994	Journal contribution	Pawlak, M.; Kuhn, A. and Vogel, H.
Synergistic instertion of two hydrophobic regions drives Sec-independent membrane assembly. J. Biol. Chem.	1994	Journal contribution	Cao, G.; Chen, S.; Whitley, P.; v. Heijne, G.; Kuhn, A. and Dalbey, R.E.
Charged residues render proOmpA potential-dependent for initiation of membrane translocation. J. Biol. Chem.	1993	Journal contribution	Geller, B., Zhu, H.-Y., Cheng, S.; Kuhn, A. and Dalbey, R.E.
Structural characterization of M13 procoat, M13 coat and Pf3 coat proteins. Biochemistry	1993	Journal contribution	Thiaudière, E.; Soekarjo, M.; Kuchinka, E.; Kuhn, A. and Vogel, H.
Bacteriophage T4 Gene 21 encodes two proteins essential for phage maturation. Virology	1992	Journal contribution	Hintermann, E. and Kuhn, A.
Distinct domains of an oligotopic membrane protein are Sec-dependent and Sec-independent for membrane insertion. J. Biol. Chem.	1992	Journal contribution	Lee, J., Kuhn, A. and Dalbey, R.E.
Distinct steps in the insertion pathway of bacteriophage coat proteins. Membrane Biogenesis and Protein Targeting	1992	Book chapter	Kuhn, A.., Troschel, D.
Use of site-directed mutagenesis to define the limits of sequence variation tolerated for processing of the M13 procoat protein by the E. coli leader peptidase. Biochemistry	1991	Journal contribution	Shen, L.M., Jong-In, L., Cheng, S., Jütte, H., Kuhn, A. and Dalbey, R.E.
Bacteriophage M13 and Pf3 tell us how proteins insert into membrane. J. Struct. Biol.	1990	Journal contribution	Kuhn, A., Rohrer, J. and Gallusser A.
Efficient translocation of positively charged residues of M13 procoat protein across the membrane excludes electrophoresis as the primary force for membrane insertion. EMBO J.	1990	Journal contribution	Kuhn, A., H.Y. Zhu and R.E. Dalbey
Initial steps in protein membrane insertion. Bacteriophage M13 procoat protein binds to the membrane surface by electrostatic interaction. EMBO J.	1990	Journal contribution	Gallusser, A. and Kuhn, A.
The function of a leader peptide in translocating charged amino acyl residues across a membrane. Science	1990	Journal contribution	Rohrer, J. and Kuhn, A.