Detection, production, and application of microbial arylsulfatases

Publikations-Art

Zeitschriftenbeitrag (peer-reviewed)

Autoren

Stressler, T.; Seitl, I.; Kuhn, A.; Fischer, L.

Erscheinungsjahr

2016

Veröffentlicht in

Applied Microbiology and Biotechnology

DOI

10.1007/s00253-016-7838-4

Arylsulfatases are enzymes which catalyze the hydrolysis of arylsulfate ester bonds to release a free sulfonate.They are widespread in nature and are found in microorganisms, most animal and human tissues, and plant seeds. However, this review focuses on arylsulfatases from microbial origin and gives an overview of different assays and substratesused to determine the arylsulfatase activity. Furthermore, the production of microbial arylsulfatases using wild-type organisms as well as the recombinant production using Escherichia coli and Kluyveromyces lactis as expression hosts isdiscussed. Finally, various potential applications of these enzymes are reviewed.