Analyse von Präbiotika in Milch: Laktose, Lactulose und Epilactose

Publikations-Art
Zeitschriftenbeitrag
Autoren
Kuschel, B.; Seitl, I.; Stressler, T.; Fischer, L.
Erscheinungsjahr
2017
Veröffentlicht in
Biospektrum
DOI
10.1007/s12268-017-0877-2
Abstract

Lactulose and epilactose are potentially prebiotic isomers of the milk sugar lactose. Enzymatically they can be produced using cellobiose 2-epimerases. These sugars are notoriously hard to quantify due to their chemical similarity, which results in incomplete separation. With a new commercial stationary phase we achieved baseline separation on a basicHPLC system. The method allows the parallel quantification of lactose, epilactose and lactulose within a wide range of concentrations befitting most food samples.

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